Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIAGEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARILEEGLGKLANVERLILQPNN--------REDDLRIWLQDHGFQIVAERILEEAGKFYEILVVEAGQMKLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIKEVLHVSK 3A27 Chain:A ((113-246)) --------MAFISNENEVVVDMFAGIGYFTIPLAKYSKPKLVYAIEKNPTAYHYLCENIKLNKLN-NVIPILADNRDVELKDV-ADRVIMGYVHK--THKFLDKTFEFLKDRGVIHYHETVAEKIMYERPIERLKFYAEKNGYKLI---------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3A27.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 606 -63656 -105.04 -505.20 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -105.04 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_3A27.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A27-query.scw PDB file : Tito_Scwrl_3A27.pdb: