Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIEQLQSKIEQARTLYKEFRLYDLGSFALNRFTPKDGFEQVLNVRYGLKPRHRLDIYRSTKRLAHRPLIVFVHGGAWQHGNKRD-----YLFIGEAFTKEGYDVAVINYQLAPKNIFPSYVDDLTQALNYLHQNQEKLEISTENIVLMGHSAGAFNVMSAVYHPKPNTIQCLGNIKAIFGLAGPYHFDYKGDP-LAEDAFDQSISYK-EVMPYYFVNQNHIKHYLLMAENDQLVKKEN-TFDLDQALRQSGN-HSHIAVIPKTGHITILASLSSFVSHYFRTKRTILHFLDEVF 4ZRS Chain:B ((58-304)) ------------------------------------------TRDLEYGEDPRHRLDLFRKAD-TRDAPVLVFVHGGGFVMGDKRLAETPFYDNIGVFAAQQGFVGVTITYRLAPAHQFPSGPEDLAAVVRWLKANVAQYGGDPDKIVLSGQ-AGAAHVASYIAHKAHHATEG-GGIAGAILMSGIYDTLTATPNEFLIAYYGDDPKGWGPASSMAGLINTEIPLMLTVSEFDPED-FQRQAAQFVCAWGMAHAAYPEMHYLVGHNHLSPAQSIG---TEIKAIGRMVAGFVRRV-

General information: TITO was launched using: RESULT: Template: 4ZRS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1367 -7759 -5.68 -32.74 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -5.68 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_4ZRS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRS-query.scw PDB file : Tito_Scwrl_4ZRS.pdb: