Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------MSQSSAALNSPNPMKKKVITALI-------AVAIGHIGVLWAVSHIKPAELKPIDKKPLQVRFVKIQEQPKPLPP-KPKEPPKKPEPK----KEVKEVKVVEKP--VTPPKKVEKIQQVK------------KAETPKETVKT-------EPKVETKVVTTTTTVTEKVVEKPKPVVQEAPKAQPAPDPSPKRVSIGGSGVQWSRSPRLTVSPKDLQGEARSVVVLIEADEKGK----IINVRVTRSSGISSLDDKVVRAVRGAKFKP----------YMENGVAYPIRAEQPFDLNP---------------- 4NTC Chain:A ((2-326)) MSIGKLLSNGALLVDVLIIGAGPAGLSTATGLARQLHTAVVFDSGVYRNAKTQHMHNVLGWDHRNPAELRAAGRADLTTRYSTIQFQNSTIEAIRQVETNQLFEARDNEGHSWYGRKVVLATGVRDIPLDIEGYSECWANGIYHCLFCDGYEERGQETVGVLALGPIANPARALHLARMALRLSESVTIYTNGNEQLAKEIQQAAEESP----VGASGLKFEARPIRRFEKGDV---AKTVIVHLGESESKTEGFLVYNPQTEVNGPFAKQLALNMTEGGDILTTPPFYETSVPGVFAVGDCATPLKAVTPAVSMGSLAAGGLVAQLQAQAL

General information: TITO was launched using: RESULT: Template: 4NTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1101 77865 70.72 319.12 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 70.72 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.085

(partial model without unconserved sides chains): PDB file : Tito_4NTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NTC-query.scw PDB file : Tito_Scwrl_4NTC.pdb: