Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISLLGSGRVAFHLAKALLAQGHHIAQVYARDFEKTKKFAEQI----------Q---------AKA-CQ-SLQEFQPTDLIILAVSDSA--ITELATQVHELF-PKMLMVHTSGSTDIQVISQVH---EKAGVFYPLQTFSLERDVDWKATPLFVEA--TNKDDLVILSDLANSLSKRVYQYTSKQRLTLHLAAVFACNFSNYCFDMAKQVVDAEQVDFGLLYPLILETAKKATENDPKQMQTGPAMRGDQNILVMHQSLLAQANRDDLKQVYQLLSDGIVKRHHSS 1ZCJ Chain:A ((297-536)) SSVGVLGLGTMGRGIAISFARVGISVV-AVESDPKQLDAAKKIITFTLEKEASRAHQNGQASAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALNVDDIASSTDRPQLVIGTHFFSPAHVM------RLLEVIPSRYSSPTTIATVMSLSKKIGKIGVVVGNCYGF-------VGNRMLAPYYNQGFFLLEE-GSKPEDVDGVLE-EFG---------FKMGPFRVSDLAGLDVGWKIRKGQ-----------------------

General information: TITO was launched using: RESULT: Template: 1ZCJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1119 -92065 -82.27 -436.32 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -82.27 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_1ZCJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZCJ-query.scw PDB file : Tito_Scwrl_1ZCJ.pdb: