Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSQEPNQSLFNQASTSIQACIECMFACKHCASACLQEEHVHMMRKCIKHCLTCVETCQLCTSLELRHS----ELAEQAMQLCANACQLCAAEC---SKHEHEHCQVCAKACLACAQACQAYRA 5FIG Chain:F ((4-85)) -------------------------------------------YSEACIEACIDCMKACNHCFTKCLEE-QHHLSGCIRLDRECADICALAVKAMQTDSPFMKEICALCADICEACGTECGKHD-

General information: TITO was launched using: RESULT: Template: 5FIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 204 -31930 -156.52 -437.40 target 2D structure prediction score : 0.84 Monomeric hydrophicity matching model chain F : 0.67 3D Compatibility (PKB) : -156.52 2D Compatibility (Sec. Struct. Predict.) : 0.84 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.636

(partial model without unconserved sides chains): PDB file : Tito_5FIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FIG-query.scw PDB file : Tito_Scwrl_5FIG.pdb: