Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVVKAKNLIAFRIWLEKLGYSVKNLADGKGFTFSLKKEYGLV---------TCDLAGNALAMQLGEEFEDHLKA 3OPN Chain:A ((165-222)) ----------EKVLKTATQLGFSVKGLTFSPIKGGAGNVEFLVHLLKDGKAEIAQQVNIESVLQKESE--------

General information: TITO was launched using: RESULT: Template: 3OPN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 130 -8153 -62.72 -166.39 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -62.72 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.174

(partial model without unconserved sides chains): PDB file : Tito_3OPN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OPN-query.scw PDB file : Tito_Scwrl_3OPN.pdb: