Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILVLGSGGREHALAWKIAQDAKVTQVFVAPGNAGTATEPKCVNVQLDILDNPAIIAFAKENNVELIVVGPEAPLVNGVVDAAREAGVKIWGPTQYAAQLEGSKAFAKHFLKRHNIPTAFYEVFTEVDAAKAYVEKNGAPIVIKADGLAAGKGVIVAMTNEEAFAAIDDMLAGNKFGDAGSRVVIEQFLAGEEASFICMIDGKNILPMATSQDHKRIFEGDQGPNTGGMGAYSPAPVVTPDVFERVMNEIMRPTVDGMAADGHVYTGFLYAGLMIDEQGQPRVIEFNCRFGDPETQPIMMRLKSSLVDLVEAGIAGNLPSEAEWDERKSIGIVL---AAEGYPDSVRKGDVISGIGQS--PEDTKI-----FHAGTATREDGHIVTSGGRVLCVTALGDSVLEAQINALEVCGQVTFTGMQYRSDIGYRAIAREKAE 2YW2 Chain:A ((1-420)) MKVLVVGNGGREHAIAWKVAQSPLVKELYVAKGNAGIWEIAKRVDISPT--DVEKLAEFAKNEGVDFTIVGPEAPLVEGIVDEFEKRGLKIFGPNKEAAKLEGSKAFAKTFMKKYGIPTARYEVFTDFEKAKEYVEKVGAPIVVKADGLAAGKGAVVCETVEKAIETLDRFLNKKIFGKSSERVVIEEFLEGEEASYIVMINGDRYVPLPTSQDHKRLLDEDKGPNTGGMGAYSPTPVINEEVEKRIREEIVERVIKGLKEEGIYYRGFLYAGLMITK-EGPKVLEFNVRLGDPEAQPILMRVKNDFLETLLNFYEGKDVHIKEDE-RYALDVVLASRGYPEKPETGKI---IHGLDYLKSMEDVVVFHAGTKKEGNFT------VTSGGRVLNVCAYGKTLKEAKERAYEAIRYVCFEGMHYRKDIGDKAFK-----

General information: TITO was launched using: RESULT: Template: 2YW2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2570 -191521 -74.52 -467.12 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -74.52 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_2YW2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YW2-query.scw PDB file : Tito_Scwrl_2YW2.pdb: