Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKTLQFGITNFEIDFKDLVMNTMIKNKRFENDVEFRGADIKDSRAVVILLHGRRQNADTIYELAEKIGFT--DITYILPLAPELTWYPRGFMRD------------LEDNQPYLSVALERIHYIITDLKNQNIKPENIFLMGFSQGACIVSQYLWEN-PQKLGGVIAFTGGLFGPELTYTSHEGLPINGTQLIFTGGKEDSWVPEQRVRDTAALFEKLGGNVTTKIYSGRDHLVSDEEVELAKTLLNI 1AUO Chain:B ((5-214)) ----------------------------------LILQPA-KPADACVIWLHGLGADRYDFMPVAEALQESLLTTRFVLPQAPTRPVTINGGYEMPSWYDIKAMSPARSISLEELEVSAKMVTDLIEAQKRTGIDASRIFLAGFSQGGAVVFHTAFINWQGPLGGVIALSTYAPTFGDELE-L-SASQQRIPALCLHGQYDDVVQNAMGRSAFEHLKSRGVTVTWQEYPM-GHEVLPQEIHDIGAWLA-

General information: TITO was launched using: RESULT: Template: 1AUO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1030 -29182 -28.33 -149.65 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -28.33 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_1AUO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AUO-query.scw PDB file : Tito_Scwrl_1AUO.pdb: