Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIVERPSYTSKDVEVTSREPLFSGFIQVEKVSLRHRLFNQSEYTPVLQRELVHRPEAAGVLLYNDQKQQFALIEQFRVGALDDSHSPWQL-EIIAGVLDGNESPESCIRCESLEESGCEVQDLEHLFSFYPSAGACSELFHLYVAETN----LPAVGGVFGVDNEGENIQLHLFSYSEIQTLLNSGRLRNAPVIMALQWLAQHSKTIINPKR 3O69 Chain:B ((38-184)) ------------------------------------------------KREVYDRGNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIESCAGLLD-NDEPEVCIRKAAIEETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGV-----EDEDIEVLELPFSQALEMIKTGEIRDGKTVLLLNYL------------

General information: TITO was launched using: RESULT: Template: 3O69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 645 -16386 -25.40 -115.39 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -25.40 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_3O69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O69-query.scw PDB file : Tito_Scwrl_3O69.pdb: