Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINCGAKMLIQRGGLKVVAGLGISGV-SAVNFLHEQGYQVAVTDSRPTPPGHDQIPA-GVKTSFGQLDQELLLQAEEIILSPGLAPQLPEIQAAIAKGISVVGDIQLLRRAT-DVPIVAITGSNAKSTVTTLIGLMAKDAGKKV-AVGGNLGRPALDL-LKDQPELLVLELSSFQLETTSHLNAEVAVVLNMSEDHLDRHGNMLGYHQAKHRIFQGA---KKVVFNRDDALSRPLVPD-TTPMQSFGLNAPDLNQYGVLR-D--ADGTLWLARGLQRLIKSSDLYIQGMHNVANALACLALGEAIGLPMESMLETLKQFKGLEHRCEYVKT---------VHDVRYYNDSKGTNVGATLAAIDGLGAAIEVKKGKVALILGGQG--KGQ-DFSPLRSSIEKYAKVVVLI-----GE------DAPVIEQAIQGA--TKILHAATLKEAVELCQRETQAEDVVLLSPACASFDMFKSYNDRGQQFVACVNSLV 4HV4 Chain:B ((18-471)) --------EMRRVRHIHFVGIGGAGMGGIAEVLANEGYQISGSDLAPNSV-TQHLTALGAQIYFH-HRPENVLDASVVVVSTAISADNPEIVAAREARIPVIRRAEMLAELMRYRHGIAVAGTHGKTTTTAMLSSIYAEAGLDPTFVNGGLVKAAGTHARLGSSRYLIAEADESDAS-FLHLQPMVAIVTNIEAD------DFENLKQTFINFLHNLPFYGRAVMCIDDPVVRELLPRVGRHITTYGFSDD--ADVQIASYRQEGPQGHFTLRRQDKPLIEVTLNAPGRHNALNAAAAVAVATEEGIEDEDILRALVGFQGTGRRFDFLGNFPLAPVNGKEGSAMLVDDY-GHHPTEVDATIKAARAGW--PDKRIVMLFQPHRYTRTRDLYDDFANVLSQ-VDVLLMLDVYAAGEPPIPGADSRALCRTIRNRGKLDPILVPDSESAPEMLAQILNGEDLILVQGAGNI----------------------

General information: TITO was launched using: RESULT: Template: 4HV4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2293 -5185 -2.26 -12.65 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -2.26 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_4HV4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HV4-query.scw PDB file : Tito_Scwrl_4HV4.pdb: