Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCCFVVYGGVLNMSDKKTSLVIC-FTPLQMLIAEKIVAQQPSVDLIVIALNNNDKYKYYYNIFQHPNIAKQYYLFDNNKSRFFKFINIIKFNEN--FKLEKFYENIYISSIDNK----YTHNILSKVNFNNLYTYDDGTANIVKNSIY---FKQTFESKLKDIVLKLIGIRFNLNKIKKLSRKHYTIYKGIQNIVEQTEYVSIIEKKEEDRNIFIDQEIKIFLGQPLKDIDKNFDTLALKKLL---VMEEVNYHFKHPRETDEAFF---EEIKTSYIFEDFFAKELSKYRKVIIYT-LSSTAALNIISLDNVEIRLIKNNTVEIKYPDLVQLFIKSGATVIDMDSIK 4NTC Chain:A ((2-326)) ----MSIGKLLSNGALLVDVLIIGAGPAGLSTATGLARQLHTAVVFDSGVYRNAKTQHMHNVLGWDH-RNPAELRAAGRADLTTRYSTIQFQNSTIEAIRQVETNQLFEARDNEGHSWYGRKVVLATGVRDIPLDIEGYSECWANGIYHCLFCDGYEERGQETVGVLA-----LGPIANPARALHLARMALRL----SESVTIYTNGNEQLAKEIQQAAEE---SPVGASGLKFEARPIRRFEKGDVAKTVIVHLGESESKTEGFLVYNPQTEVNGPFAKQLALNMTEGGDILTTPPFYETSVPGVFAVGDCATPLKA----VTPAVSMGSLAAGGLVAQLQAQAL-

General information: TITO was launched using: RESULT: Template: 4NTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1727 -16944 -9.81 -55.01 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -9.81 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_4NTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NTC-query.scw PDB file : Tito_Scwrl_4NTC.pdb: