Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLNKLMLGLGLTVASVAANAAGYGVIDLAKVVESSTYLKQQNASLNQSVKPTTTKLEQLGKELERLQRQAQTQGQKMKEDEIKKLQSQYQSKLNEFNSTQQGLQSRVQTSLQSMNTTFETRVKQAAEQLRKENNLDFILNKNSTVAYDAKYDLTDKMIQKVNSMK 1U2M Chain:C ((49-130)) ------------------------------------------EFKGRASELQRMETDLQAKMKKLQSMKA---GSDRTKLEKDVMAQRQTFAQKAQAFEQDR---ARRSNEERGKLVTRIQTAVKSVANS-------------------------------------

General information: TITO was launched using: RESULT: Template: 1U2M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 86 2542 29.56 31.00 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.59 3D Compatibility (PKB) : 29.56 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.657

(partial model without unconserved sides chains): PDB file : Tito_1U2M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U2M-query.scw PDB file : Tito_Scwrl_1U2M.pdb: