Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSFMQQS-----------RLKVI----LNMRHELREIFIKGCDDKIEKKGDNVGLSFYAFFKNKNDNPELL-----MEAAHWWIME-----HKLDHFEKAVKIKQLVLSEH-------------- 5KNW Chain:A ((1-125)) MHHHHHHSNATGPQFVSGVIVKIISTEPLPGRKQVRDTLAAISEVLYVDLLEG-DTECHARFKTPEDAQAVINAYTEINKKHCWKLEILSGDHEQRYWQKILVDRQAKLNQPREKKRGTEKLITKA

General information: TITO was launched using: RESULT: Template: 5KNW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 296 22824 77.11 265.40 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 77.11 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_5KNW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KNW-query.scw PDB file : Tito_Scwrl_5KNW.pdb: