Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDVIKDLSELRLSYEQGELYETQVASNPHEQFLGWFNHALAANLHEPYAMSLATASASGRPHVRTVLLRGATEAGYDFYTNYDSQKGIDLAENPYAELLFYWPSLERQVRVGGHVVKIPEQESTDYYHKRPRDSQIAAYISTPQSGKIESRELLQQRFQDLQQQVQSHEVLDKPEFWGGYRLQPDYYEFWQGRPNRLHDRLSYEKIDGQWTLHRLMP 4HMW Chain:B ((28-215)) --------------------------SEPITLLKRWLATADVARVREPKALALATATSDGRISSRVIAFSSIDDRGVIFCTHSTSRKGRELTETGWASGLLYWRETGQQIMISGQAVPLEESENDKLWFGRSVPMH-AMSSASHQSDELVDREALRAHAAEL---LALGVALPRPPRFVGYRLEPHEMEFWAASSDRLHRRLRYERDGNDWKTTQLQP

General information: TITO was launched using: RESULT: Template: 4HMW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 790 -55027 -69.65 -292.70 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -69.65 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_4HMW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HMW-query.scw PDB file : Tito_Scwrl_4HMW.pdb: