Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDILENPLELCGFAFIEFVSKE--NELDPIFETIGFSKVAKHKSK--KAYLWRQGNINIILNYQPES--YASFFFNEHGPSACAMGFKTRDAAKAFKKAVELGAEPMYSKVGPMELNIPAIKGIGGMPIFLVDCDIY--ENDFVFFDDAQRNPEGAGLKEIDHLTHNVYKGRMEYWANFYEKIFNFQEIRYFDIKGEYTGLTSKALTAPDGMIRIPLNEDSDK-GNGQIAEFLADFNGEGIQHIAFICDDLISTWDKLKGLGLKFMTPPPNTYYEMLEERLPEHGEPTEELKQRCILLDGSTKDGQKKLLLQIFSENMIG--PVFFEFIQRKDDDGFGEGNFKALFESIERDQIRRGVLEAK 2R5V Chain:B ((2-348)) --------QNFEIDYVEMYVENLEVAAFSWVDKYAFAVAGTSRSADHRSIALRQGQVTLVLTEPTSDRHPAAAYLQTHGDGVADIAMATSDVAAAYEAAVRAGAEAVRAPGQH-AVTTATIGGFGDVVHTLIQR---ELPPGFTGSM--------VDLLGIDHFAICLNAGDLGPTVEYYERALGFRQIFDEHIVVGAQAMNSTVVQSASGAVTLTLIEPDRNADPGQIDEFLKDHQGAGVQHIAFNSNDAVRAVKALSERGVEFLKTPG-AYYDLLGERITLQTHSLDDLRATNVLADEDH----GGQLFQIFTASTHPRHTIFFEVIERQGAGTFGSSNIKALYEAVELERTG-------

General information: TITO was launched using: RESULT: Template: 2R5V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1863 62125 33.35 194.75 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : 33.35 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_2R5V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R5V-query.scw PDB file : Tito_Scwrl_2R5V.pdb: