Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRAISHIDTFQGLILALQNYWAEQGCVVLQPYDMEM---------GAGTFHTATFLRALGPE--T-WNAAYVQPSRRPK----DG-RYGENPNRLQHYYQFQVVLKPNP------DNIQQLYLDSL-KAIGIDTL------------------------THDIRF--VEDNWES-PTLGAWGLGWEVWLN----------GMEVTQFTYFQQV----GG-VECYPVTGEITYGLERLAMYLQGVDSVYDLVWTKGQFGTVTYGDVFHQNEVEQSTYNFEYAPVDKLFELFDFYESEANRLMEAKLPLPAYEQVVKASHTFNLLDARGAISVTERQRYILRVRTLARAIAQSYVQARAELGFPMAEPHLRDEVLAQLKAQAESEAAKAEKN 1YFR Chain:A ((2-248)) -------SLSAHEIRELFLSFFEKKGHTRVKSAPLVPENDPTLLFVNAGMVPFKNVF--LGLEKRPYKRATSCQKCLRVSGKHNDLEQVGYTS----RHHTFFEMLGNFSFGDYFKKEAIEYAWEFVTEVLKLPKEKLYVSVYKDDEEAYRIWNEHIGIPSERIWRLGEEDNFWQMGDVGPCGPSSEIYVDRGEEYEGDERYLEIWNLVFMQYNRDENGVLTPLPHPNIDTGMGLERIASVLQGKNSNFEI--------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1YFR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 652 -11093 -17.01 -64.49 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -17.01 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_1YFR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YFR-query.scw PDB file : Tito_Scwrl_1YFR.pdb: