Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKDERIIQNQDNKKTIRHEDYIAGIGKGMAILDSFSSNQHRLKISMAAEKTGLTRAAARRHLLTLEYLGYLESDG-HYYYLTPKVLKFSGAYLGAAQLPKVSQPLLNLLTNQTSLIYSVMVLDGYEAITIARSAAHQQTDRVNPYGLHLGNRLPAHATSAGKILLAHLSQEEQLDWLQKYPLQRLTKYTHVENDKFLELLKEIREQDWCYSREEHELGFHALAVPIYGQDFKVVAALNIVSPTMRTTEGYLIQYILPLLQETARDLRQVL 5H1A Chain:D ((26-265)) --------------------SGDSMLARVVRVLETFNVDRTAQTASDIGRRAALPSSTAHRVVDEMVLVGILERGIDGKVRLGMRLWELALRGSMALRLRQVALPHMERVQQRVREHTQLAVLEHNEVLFLERLSHH----EAVSNLARVAGRLPVHASSSGLMLLAHAGPEVREEVLSK-PLPRVGPGTVTDPEALRRLLANAYRAGYVAAPGYIEAVATGIAVPIRS-EGVVIAALSAVQPLQNAV-----EPTVEILREAAVGIETDL

General information: TITO was launched using: RESULT: Template: 5H1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1092 -68613 -62.83 -287.08 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.72 3D Compatibility (PKB) : -62.83 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_5H1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H1A-query.scw PDB file : Tito_Scwrl_5H1A.pdb: