Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAIPTLTPLAMPCNSSFEAKDWEILSSHWYPVARIQDVSTAPQRVKLLDVNMALYKTESGEIHLVRDICPHRGVPLTKGWVDGEEIVCPYHGLRYNTEGKCTQIPAQPELTKISDRFSLTKFPVVQRYGLIWTSIHGRDIAKANIPVLDTWDDAEHQAILPPFVDIGGSSGRQLEGFIDVAHFAWVHHNAFANRDNPIVPKYHTERTNYGLKTVYISNVSNYPHELKHLEPEGFLWKRTFEVYPPFSAVLTVDFPEDGILKILNACCPISSNKTRLFVPLTRNFDQTGDLEKVYAFNAQIFAEDQDMVESQKPEELPLDLMMEAHFEADRSSTTYRRILAEWGLSKRYTV 3GL0 Chain:C ((9-134)) -----------------------------WYVAALPEELSEKPLGRTILDTPLALYRQPDGVVAALLDICPHRFAPLSDGILVNGHLQCPYHGLEFDGGGQCVHNPHGNGARPAS--LNVRSFPVVERDALIW--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GL0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 481 -61665 -128.20 -604.56 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.58 3D Compatibility (PKB) : -128.20 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_3GL0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GL0-query.scw PDB file : Tito_Scwrl_3GL0.pdb: