Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFLTKFVKPMQPYILVLYYSKYGSTKEMAHLIANGVESAG-MNVKIRTVPNIATVVTEAEPSIPEEGDI-YCTLEELANCAGLALGSPTRFGNMASEMKYFWDQTTNLWLNGALHNKPACVFTSSGSMHGGQESTLLTMLPPLFHHGMMILGLPNSIPALSNTK--TGGTPYGASHVSGPRHDQSLSQDEKNLCEAQGKRLGEIVKKLYS 4LAF Chain:D ((5-203)) --------------IQIVFYSSYGHIYKMAEAIAAGAREVGDVEVTLLQVPEL-------------FGSIPYATPEVLAEADAIIFGTPTRFGNMCSQMRNFLDQTGGLWMSGGLIGKVGSVFTSTASQHGGQETTITSFHTTLLHHGMVIVGVPYSEPGLTNMTEISGGTPYGASTLAGADGSRQPSENELQIARFQGKHVATIAKRL--

General information: TITO was launched using: RESULT: Template: 4LAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1003 -23022 -22.95 -129.34 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -22.95 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_4LAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LAF-query.scw PDB file : Tito_Scwrl_4LAF.pdb: