Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKDIKVDPAQLEAALDARLKIRAG-FDKPTAGMAAGMTQVNMISVPRDWAYDFLLYAHRNPQSCPVLDVLEEGIYATKLAADSDIRTDFPRYRIWKDGEMVDEVTDAREIYNAHPDLVTFLIGCSFSFETALQEAGIEVRHIHDDTNVPMYLSNIKCEPAGRISGNMVVSMRPIPSHQISEAVKITARMPSVHGAPVHIGHPESLGIKDVNKPDFGDASRIEAGEIPVFWACGVTPQAAVINSKIPFAISHAPGYMFITDIPDRAWMG 2PIF Chain:B ((9-263)) --------QSAIAAAREARGTYRNGLVT-PTAGVAPGMTQANLIALPRDWAYDFLLYAQRNPKACPILDVSDAGSPTTLLAEGSDLRTDIPMYRIWRDGKLAEEVSDATQAWAEHDDMVAFLIGCSFTFETPLQEAGIEVRHITDGCNVPMYRTNRACRPAGRLHGEMVVSMRPIPADRVAEASAISGR----HGAPVHIGEPGRLGINDLSRPDFGDAVSIKPGEVPVFWACGVTPQAAVMASGVPFAITHSPGYMFITDVPD-----

General information: TITO was launched using: RESULT: Template: 2PIF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1416 -49319 -34.83 -197.28 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.88 3D Compatibility (PKB) : -34.83 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_2PIF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PIF-query.scw PDB file : Tito_Scwrl_2PIF.pdb: