Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDLFLNRKSFVVKSLAITVTALMMSGANAATSDKEEIRKLRQEVEALKALVQEQRQVQQQQQQVQQQQQVQLAEVKAQPQPAAAPASPLASLKSKTGADVNLYGFVRGDANYIIEGADNDFADVSKSNGKTQDKLRATAKTTRLGLDFTAPVS-GAKVGGKIELDFASSDNDKSENVRIRHAYLTYN----NWLFGQTTSNFLS-SHAPEMIDFS--TN----------I---GGGTTRIPQVRYNYKLAPATQLFVSAEEGNSSATGDSVKYRLPVLTAKVTQGYADGNGNASARAFVENYKSGA---GDDKTGWGIAAGTDFKVSDPLKLFADASYVVGNSNYLYGSNSAYTLVNDNIEQNEFVAVQVGGTYKILPNLRSTLAYGAQFADDGTDYAKNNVTANEKVQQAWINFIYTPVKPIDLGVEYVNGKRDTFEG--------------KSYKDNRVGLMAKYSF 2FGR Chain:A ((2-332)) --------------------------------------------------------------------------------------------------SSVTLFGIVDTNVAYVNKDAAGD---------SRYGLGTSGASTSRLGLRGTEDLGGGLKAGFWLEGEIFGDDGNASGFNFKRRSTVSLSGNFGEVRLGRDLVPTSQKLTSYDLFSATGIGPFMGFRNWAAGQGADDNGIRANNLISYYTPNFGGFNAGFGYAFDEKQTI-GTADSVGRYIGGYVAYDN--GPLSASLG--LAQQKTAVGGLATDRDEITLGA--SYNFG-VAKLSGLLQQTKFKRD--------------IGGDIKTNSYMLGASAPVGGVGEVKLQYALYDQKAI----------DSKAHQITLGYVHNLSKRTALYGNLAFLKNKDASTLGLQAKGVYAGGVQAGESQTGVQVGIRHAF

General information: TITO was launched using: RESULT: Template: 2FGR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1607 54562 33.95 186.22 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 33.95 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_2FGR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FGR-query.scw PDB file : Tito_Scwrl_2FGR.pdb: