Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKHQFKLSDPRLLSRIGYQFKQPELLQLALTHRSVSHKYNYERLEFLGDSLLGMIIANYLYHAYPHENEGRLTRMRATLVRQEALGKIATDLQLSRCLILSTGELKSGGHHRESILADTVEAIIGAIYLDSS-DLNLLKDIVLKWYTP-YLDHIEPTDQLKDPKSRLQEYLQARKKPLPVYEVVDIQGDAPHQHFKVECLVDGLSKIHGEGASRRFAEQAAAAEILKLLEQ 1YYO Chain:B ((6-219)) -----------QLEKKLGYTFKDKSLLEKALTHVSYSKKEHYETLEFLGDALVNFFIVDLLVQYSPNKREGFLSPLKAYLISEEFFNLLAQKLELHKFIRIKRGKI------NETIIGDVFKALWAAVYIDSGRDANFTRELFYKLFKEDILSAIKEGRVKKDYKTILQEITQKRWKERPEYRLISVEGPHHKKKFIVEAKIKEYRTL-GEGKSKKEAEQRAAEELIKLLEE

General information: TITO was launched using: RESULT: Template: 1YYO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 871 -42173 -48.42 -198.93 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -48.42 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1YYO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YYO-query.scw PDB file : Tito_Scwrl_1YYO.pdb: