Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIENIGGMNMVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHELGVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQTSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILMAKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNLNEHGNIRRAILGENIGTTVRGK 2BND Chain:B ((7-239)) -------------PVYKRILLKLSGEALQGTE-FGIDASILDRMAQEIKELVELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNARLMSAIPLNAVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEANVVLKA-TKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVFNMNKPGALRRVVMGEKEGTLI---

General information: TITO was launched using: RESULT: Template: 2BND.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1318 -178927 -135.76 -771.23 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -135.76 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2BND.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BND-query.scw PDB file : Tito_Scwrl_2BND.pdb: