Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTKLLQAIYRDSDLSEKTA-SMIEQKVSVYQQKKG------QAGKLHWDEE--DIFLITYGDQFFEEQQPTLRTFKKFYQK--YLADT-FRVVHFLPFFPYT---SDDGFSVVNYQAINDRLGTWEDIEEMRSET-----RLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT-------DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLEKGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKDNISYFGNG---ENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLASHDGIGLNPVRG------ILPEKTILELVEQLKK-----EGARVNYKKNPDGTTSPYEINATYL--DALSSPT--DTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVE---KTGENRKINRQKFLLADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSAD-SFISFIREAKEEKVLVIHNFSNQSAGYLLPKG---DYL--DLFSGQIIHCSQERKLIFQPYEYMWLKIIK 3CZG Chain:A ((39-638)) --LDALHALYGQRADYASWLAQWLGEVGDIARQRPQALQTLDSTRHAGWFGQPHMLGYSAYADRFA-------GTLQGVAERVPYLQELGVRYLHLLPFLRARAGDNDGGFAVSDYGQVEPSLGSNDDLVALTSRLREAGISLCADFVLNHTADDHAWAQAARAGDARYLDYYHHFADRTVPDRYEATLGQV-------GNFTWVDDTA--QWMWTTFYPYQWDLNWSNPAVFGDMALAMLRLANLGVEAFRLDSTAYLWKRIGTDCMNQSEAHTLLVALRAVTDIVAPAVVMKAEAIVPMTQLPPYFGSGVDEGHECHLAYHSTLMAAGWSALALQRGDILHNVIAHSPPLPRHCAWLSYVRCHDDIGWNVLQHEACGNAAQPPFSLRDVARFYANAVPGSYARGESF-----GVHGTNGMAAALAGIQAAQEAGDAAALAVAVDRLVLLYAIALAMPGVPLIYMGDELAMVNDPGYRDDPHRQHEGRWLHRPAMDWQLAAQRHDAKSLSGTVYRRLRGLIRQRAALGALAADQALASIALNDPRVFALTRG---DSFIALHNFSDQLLDVELAAIGVDGWTLL--------------SIVLPPYGVRWLQRG-

General information: TITO was launched using: RESULT: Template: 3CZG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2936 -59879 -20.39 -115.15 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -20.39 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_3CZG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CZG-query.scw PDB file : Tito_Scwrl_3CZG.pdb: