Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---VRICSLALGKIIN---------GEVTEYLEDTLQQLPGPAGN-------VVLEYED--FSIQLSLTKNCWPKLK-------RGS----VVPKGYSLHVYSPTIEGHAATSAVLEGDDRLMLPGFDPLKMRVQFYEWLVGLSWKPLKKGP- 5I2X Chain:A ((1-150)) SGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEEFGFRGTEGRLLLLQDCGVHVQVA--EGGAPAEFYLQVDRFSLLPTEQP-RLRVPGCNQDLDVQKKLYDCLEE

General information: TITO was launched using: RESULT: Template: 5I2X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 444 9873 22.24 84.38 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 22.24 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_5I2X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I2X-query.scw PDB file : Tito_Scwrl_5I2X.pdb: