Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WTGKSEASNRPPPGRRPEETTWFTSDPSYACGVKIFRPGSEGS--SGLAEI---THLNFHEKIK---VLKGQYECEVGPSCSTLRCTMPRGWRLDIFGKSFPTRRTTYFAENGNPEYNTLASEANI--------------PLYDCRVVIRFPPTGGRDTGPLAEATHFNLGEVVG---VLGGRHTCQAQGQC-----ANVNCNVPKGYTYYSLT-GGPFQT-KEKSHTFQSDA 1HV9 Chain:A ((252-452)) ------VMLRDP------ARFDLRGTLTHGRDVEI----DTNVIIEGNVTLGHRVKIGTGCVIKNSVIGDD---CEISPYTVVEDANLAAACTIGPFARLRP---GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANKFKTIIGDDVFVGSDTQLVAP--VTVGKGATIAAGTTVTRNVGEN----ALAISRVPQTQKEGWRRP----

General information: TITO was launched using: RESULT: Template: 1HV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1064 14449 13.58 85.50 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 13.58 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_1HV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HV9-query.scw PDB file : Tito_Scwrl_1HV9.pdb: