Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence RPYHGRIQDLRRPLLCDVYIEYPSGQVFDQALGHDIVPAGETAEI---SGFKCKTVRGTCVILPTCDTPPEYRVTSWKFEREMSDPERTRFIAR---FPNVRI 1JJC Chain:B ((400-474)) ---------PPEAIPFRP-------EYANRLLGTSY-PEAEQIAILKRLGCRVEGEGPTYRVT-----PPSHR---LDLRLE---EDLVEEVARIQGYETIPL

General information: TITO was launched using: RESULT: Template: 1JJC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 269 -6564 -24.40 -95.13 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -24.40 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_1JJC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JJC-query.scw PDB file : Tito_Scwrl_1JJC.pdb: