Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -NCSVQVLDANNFQIGTACIPKGGQGSVRYNGGSYFITATSSCGLGFVSTQTHPAGWQLKLLGNC---------- 1EH9 Chain:A ((491-557)) CDRRVNVVNGENWLIIKG----REYFSLYVFSKSSIE-VKYSGTLLLSSNNSFPQ--HIEE-GKYEFDKGFALYK

General information: TITO was launched using: RESULT: Template: 1EH9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 160 2063 12.89 36.84 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 12.89 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.329

(partial model without unconserved sides chains): PDB file : Tito_1EH9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EH9-query.scw PDB file : Tito_Scwrl_1EH9.pdb: