Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAACTARRALAVGSRWWSRSLTGARWPRPLCAAAGAGAFSPASTTTTRRHLSSRNRPEGKVLETVGVFEVPKQNGKYETGQLFLHSIFGYRGVVLFPWQARLYDRDVASAAPEKAENPAGHGSKEVKGKTHTYYQVLIDARDCPHISQRSQTEAVTFLANHDDSRALYAIPGLDYVSHEDILPYTSTDQVPIQHELFERFLLYDQTKAPPFVARETLRAWQEKNHPWLELSDVHRETTENIRVTVIPFYMGMREAQNSHVYWWRYCIRLENLDSDVVQLRERHWRIFSLSGTLETVRGRGVVGREPVLSKEQPAFQYSSHVSLQASSGHMWGTFRFERPDGSHFDVRIPPFSLESNKDEKTPPSGLHW 3NRF Chain:B ((3-97)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------APDAVMVFARQGDKGSVSVGDKHFRTQAFKVRLVNAAKSEISLKNSCLVAQSAAGQSFRLDT---VDE--ELTADT----------L-KPGASVEGDAIFASEDDAVYGAS---------------------

General information: TITO was launched using: RESULT: Template: 3NRF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 390 -40432 -103.67 -425.59 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -103.67 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.257

(partial model without unconserved sides chains): PDB file : Tito_3NRF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NRF-query.scw PDB file : Tito_Scwrl_3NRF.pdb: