Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIA--NIKESTLYPILKKLEGNSFLTTYSRE-FQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGSIRHDKN 4ESB Chain:A ((3-105)) --SQMLKGVLEGCILYIISQEEVYGYELSTKLNKHGFTFVSEGSIYPLLLRMQKEKLIEGTLKASSLGPKRKYYHITDKGLEQLEEFKQSWGMVSTTVNNLLQGE------

General information: TITO was launched using: RESULT: Template: 4ESB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 301 -2899 -9.63 -28.99 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -9.63 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4ESB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ESB-query.scw PDB file : Tito_Scwrl_4ESB.pdb: