Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVAQNTRGVQLIEHVSP---QMLKAQLESIFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY-VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGF-SIHDPEDMQRAGRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWE--SPRIQTCHTHGTGCTFAAVITAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD 4C5N Chain:C ((3-264)) ---LKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKD--TWSHDVTPLPMDVFEKQLETALS-IGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVM---------NPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKKAATIIFDK-GAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYN-

General information: TITO was launched using: RESULT: Template: 4C5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1487 3131 2.11 12.73 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : 2.11 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_4C5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C5N-query.scw PDB file : Tito_Scwrl_4C5N.pdb: