Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYLREQIYLM-------MLDFSEAERD-GMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAVLRYYQLLGDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYIVVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVSMIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFLLFRGFKKIKWSEVQSKSRCKAVFCYNEGKNVRN 5J9T Chain:B ((1-109)) ------MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSSGNIIKGFDAFNNNDRIFSLSSATYVKQQHGQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5J9T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 81 6560 80.98 95.07 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.43 3D Compatibility (PKB) : 80.98 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_5J9T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J9T-query.scw PDB file : Tito_Scwrl_5J9T.pdb: