Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MWKEIRKRGFKN-------KAFRTLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFENR------------------------------------------------------------------------------------------------------------------------------ 4O5J Chain:A ((27-400)) LKNLNEKYEQLSQYLNQVASLKQSIQNANNIELVNSSLNYLKSFTNNNYNSTTQSPIFNAVQAVITSVLGFWSLYAGNYFTFFVGKKVDSGQPASVQGNPPFKTIIENCSGIENCAMDQTTYDKMKKLAEDLQAAQTNSATKGNNLCALSGCAATSNPPNSTVSNALNLAQQLMDLIANTKTAMMWKNIVISGVSNTSGAITSTNYPTQYAVFNNIKAMIPILQQAVTLSQSNHTLSASLQAQATGSQTNPKFAKDIYTFAQNQKQVISYAQDIFNLFNSIPAEQYKYLEKAYLKIPNAGSTPTNPYRQVVNLNQEVQTIKNNVSYYGNRVDAALSVARDVYNLKSNQAEIVTAYNDAKTLSEEISKLPH

General information: TITO was launched using: RESULT: Template: 4O5J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 19 1833 96.47 34.58 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 96.47 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_4O5J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O5J-query.scw PDB file : Tito_Scwrl_4O5J.pdb: