Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEIW----------KYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS 1MPZ Chain:A ((1-41)) -----CTTGPCCRQCKLKPAGTTCWKTSLTSH----------YCTGKSCDCPLYPG-------------------

General information: TITO was launched using: RESULT: Template: 1MPZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 97 4676 48.20 150.82 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 48.20 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_1MPZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MPZ-query.scw PDB file : Tito_Scwrl_1MPZ.pdb: