Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVLGLETSCDETGLALYDSELGLRGQVLYSQ-IKLHAEYGGVVPELASRDHVRKLIPLMNQLLEQSGVKKQEIDAVAYTRGPGLMGALMTGALFGRTLAFSLNKPAIGVHHMEGHMLAPLLSSQPPEFPFVALLVSGGHTQLMVVHGIGQYELLGESIDDAAGEAFDKVAKMMNLPYPGGPNIAKLALSGDPLAFEFPRPMLHQGLDFSFSGLKTAVSVQLKKLNGENRDADIAASFQEAIVDTLVKKSVKALKQTGLKRLVIAGGVSANLRLREQLETSLARIKAQVYYAEPALCTDNGAMIAFAGYQRLKAGQHDGLAVTTTPRWPMTELTIPE 5JMV Chain:C ((6-306)) MICLGLEGTAEKTGVGIVTSD----GEVLFNKTIMY-------NPREAADHHAETFPKLIKEAFEV--VDKNEIDLIAFSQGPGLGPSLRVTATVARTLSLTLKKPIIGVNHCIAHIEIGKLTTEA-EDP-LTLYVSGGNTQV-IAYVSKKYRVFGETLDIAVGNCLDQFARYVNLPHPGGPYIEELARKGKKLV---DLPYTVKGMDIAFSGLLTAA---MRAYDAGERLEDICYSLQEYAFSMLTEITERALAHTNKGEVMLVGGVAANNRLREMLKAMCEGQNVDFYVPPKEFCGDNGAMIAWLGLLMHKNGR---------------------

General information: TITO was launched using: RESULT: Template: 5JMV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1758 -58320 -33.17 -199.04 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -33.17 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_5JMV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JMV-query.scw PDB file : Tito_Scwrl_5JMV.pdb: