Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKHEIISLRHYRIETEASAHKHRVPLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDW---GVPTRNQAK-----YNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGWSLGGALSLCYTALFKDKDIKNLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFKLTAPIGNLKTYWQLLKNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELSTGVIKFGELTAYLKDI--DCSVLAVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPSGHMGVVS--GSQAPTSVWPEMSNWLAMRSD-- 4ZWN Chain:A ((22-331)) SAPYPYKVQTTVPELQYENFDGAKFGYMFWP--VQNGTN-EVRGRVLLIHGFGEY------------------------TKIQFRLMDHLSLN-------------------GYESFTFDQRGAGVTSPGRSKGVTDEYHVFNDLEHFVEKNLSECKAKG--IPLFMWGHSMGGGICLNYACQGKHKNEISGYIGSGPLI------------------------------------ILHPHTMYN----KPTQIIAPLLAKFSPRVRIDTGLDLKGITSDKAYRAFLGSDPMSVPLYGSFRQIHDFMQRGAKLYKNENNYIQKNFAKDKPVIIMHGQDDTINDPKGSEKFIRDCPSADKELKLYPGARHSIFSLETDKVFNTVFNDMKQWLDKHTTTE

General information: TITO was launched using: RESULT: Template: 4ZWN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1721 11144 6.48 37.78 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 6.48 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_4ZWN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZWN-query.scw PDB file : Tito_Scwrl_4ZWN.pdb: