Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequence-MSLLLKVRQNVPQVQSIKTSLKKRAGQKIPHMLSNASDLFFRGSEIALSGKTPFDVIYKHEIISLRHYRIETEASAHKHRVPLVIVPPLAVNMLIYDLFPTRSLIRYFLDQGFEVYLIDW---GVPTRNQAK-----YNFGTYVKVFMPEMLKQVRIHSGQQQLSLHGWSLGGALSLCYTALFKDKDIKNLMILASPIDTHKAGYMGKLYGSLTKPAQWVRKHTPFRIRQHVPSEVFHIHGWQNTLGFKLTAPIGNLKTYWQLLKNLDNREFIVDHATSSSFIDNMLAYPGGVMRDIILRFWIDNELSTGVIKFGELTAYLKDI--DCSVLAVGGDTDIIVTADAVKPLMDLISSQDKTFKTVPSGHMGVVS--GSQAPTSVWPEMSNWLAMRSD--
4ZWN Chain:A ((22-331))SAPYPYKVQTTVPELQYENFDGAKFGYMFWP--VQNGTN-EVRGRVLLIHGFGEY------------------------TKIQFRLMDHLSLN-------------------GYESFTFDQRGAGVTSPGRSKGVTDEYHVFNDLEHFVEKNLSECKAKG--IPLFMWGHSMGGGICLNYACQGKHKNEISGYIGSGPLI------------------------------------ILHPHTMYN----KPTQIIAPLLAKFSPRVRIDTGLDLKGITSDKAYRAFLGSDPMSVPLYGSFRQIHDFMQRGAKLYKNENNYIQKNFAKDKPVIIMHGQDDTINDPKGSEKFIRDCPSADKELKLYPGARHSIFSLETDKVFNTVFNDMKQWLDKHTTTE


General information:
TITO was launched using:
RESULT:

Template: 4ZWN.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1721 11144 6.48 37.78
target 2D structure prediction score : 0.54
Monomeric hydrophicity matching model chain A : 0.66

3D Compatibility (PKB) : 6.48
2D Compatibility (Sec. Struct. Predict.) : 0.54
1D Compatibility (Hydrophobicity) : 0.66
QMean score : 0.330

(partial model without unconserved sides chains):
PDB file : Tito_4ZWN.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4ZWN-query.scw
PDB file : Tito_Scwrl_4ZWN.pdb: