Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDDISGWTEWNAHFEGIEEISSPSELHGLLTGIVCVTEAPTLEEWTQ-ILTTLNVPE-LNEEALALLTDEAEDVAHALSE-DELDYLPMLPDDEHLLQDRVQALSDWCAGVVLGFGLA--SG-HVRTDERELIEHLQDVAAVEFEDSDNDEEGESSYEELYEFVRLIPVSLSIGRKKVTVAESSLLKNFYAKSKTSTVGTADQNIVEMFTPHRPS 1IZM Chain:A ((2-171)) ----LISHSDMNQQLKSAGIGFNATELHGFLSGLLCGGLKD--QSWLPLLYQFSNDNHAYPTGLVQPVTELYEQISQTLSDVEGFTFELGLTEDE-NVFTQADSLSDWANQFLLGIGLAQPELAKEKGEIGEAVDDLQDICQLGYDEDDNEEELAEALEEIIEYVRTIAMLFYSHFN---------------------------------------

General information: TITO was launched using: RESULT: Template: 1IZM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 644 18558 28.82 113.16 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 28.82 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_1IZM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IZM-query.scw PDB file : Tito_Scwrl_1IZM.pdb: