Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRILIVTGQSGSGKSSALQVLE-DLGYYCIDNLPLAL--L----PEIVAKLDHE-------N----NLEQLALGVDVRSTRADMQEFDHVFEQLQKHGTVDVIYLTTQDQDLIARFSASRR-PHPLANRFKSLLQCIHEEKQLLIPIQ-FCATVHIDTTDKSVHDLKHILLSKLGQSDNLIVILQSFGYKHGIPLDADYVFDVRHLPNPHWDLELRRFSGLDEPVKQFLEASPQANEMFEDILHFLKKWLPAFAEGHRHYMTISIGCTGGQHRSVYMVDRLKQALEAEWSVQVLHREMKHWS 3TRF Chain:A ((5-174)) -LTNIYLIGLMGAGKTSVGSQLAKLTKRILYDSDKEIEKRTGADIAWIFEMEGEAGFRRREREMIEALCKLDNIILATGGGVVL---DEKNRQQISE--TGVVIYLTASIDTQLKRIGQKGEMRRPLFIK-NNSKEKLQQLNEIRKPLYQAMADLVYPTDDLNPRQLATQILVDIKQ------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3TRF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 631 19946 31.61 132.97 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 31.61 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_3TRF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TRF-query.scw PDB file : Tito_Scwrl_3TRF.pdb: