Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIGIPTETVVGENRVAATPETVKKLISAGHSVVIERGAGVKAAYIDSAYEQVGATITD---DAYTGSQIILKVRAPQGGE-----IQKLAANTTVVAMFDPYRNTEL-DQFANQQVSAFALELLPRTLSRAQNMDVLSSQANLAGYKSVLLAAAEYQRMFPMLMTAAGTVKPARVVIMGVGVAGLQAVATAKRLGAIVEATDLRPTAKDQVESLGGKWLDVPMSEEEQQRAADAAKNGYGWMPGEQYIKDQAAIVDKAVSNADIVITTALLPGRDAPRLIKAETVAKMKPGSVILDMAVETGGNVEGSKVGETVVTENGVKILGIPNIPATVATEASALYARNVFNFVETLFDKE-KNFAINQEDEIQKALLVTHGGQVLLKRG 1U2G Chain:B ((1-375)) MKIAIPKERRPGEDRVAISPEVVKKLVGLGFEVIVEQGAGVGASITDDALTAAGATIASTAAQALSQADVVWKVQRPMTAEEGTDEVALIKEGAVLMCHLGALTNRPVVEALTKRKITAYAMELMPR-ISRAQSMDILSSQSNLAGYRAVIDGAYEFARAFPMMMTAAGTVPPARVLVFGVGVAGLQAIATAKRLGAVVMATDVRAATKEQVESLGGKFITV--------------------------RKKQAEAVLKELVKTDIAITTALIPGKPAPVLITEEMVTKMKPGSVIIDLAVEAGGNCPLSEPGKIVV-KHGVKIVGHTNVPSRVAADASPLFAKNLLNFLTPHVDKDTKTLVMKLEDETVSGTCVTRDGAIV----

General information: TITO was launched using: RESULT: Template: 1U2G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1985 -690 -0.35 -2.01 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -0.35 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_1U2G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U2G-query.scw PDB file : Tito_Scwrl_1U2G.pdb: