Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLAQRLAAVVLTLGLSAGLVGCGFHLKGTNPTATPLVYKKLSL-ELPAKTDDLETQLKVYLTANGVQLSND----NDAYVLRVLEYTPRRQLL----NGKLTEVLLRLTVTFQIEDRQGNKITEPRTLTAARSYQYDLATVNTENQQESYLQRIVIDDLAQQITRQISANRLPKAQP 5IV8 Chain:B ((6-153)) -----------------------GWHLR-----VP-TTMKTMILQSSDPN-GPLSRAVRNQLRLNGVDLIDASTLRKDVPSLRLDGSSIQKDTASVFQDGRTAEYQMVMTVHASVLIP-GHDIY-PITTKVYRSFFDNPQAALAKDAEQDMIIQEMYDKAAEQLIRKLPSVQVADV--

General information: TITO was launched using: RESULT: Template: 5IV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 491 19244 39.19 142.54 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 39.19 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_5IV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IV8-query.scw PDB file : Tito_Scwrl_5IV8.pdb: