Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MSEEQQVQPQLALERIYTKDISFEVPGAQVFTKQWQPELNINLSSAAEKIDPTHFEVSLKVVVQANNDNETAFIVDVT------QSGIFLIDNIEED---------RLPYILGAYCPNILFPFLREAVNDLVTKGSFPQLLLTPINFDAEFEANMQRAQAAAVEGQA 1P32 Chain:A ((74-282)) MHTDGDKAFVDFLSDEIKEERKIQKHKTLPKM-SGGWELELNGTEAKLVRKVAGEKITVTFNINNSIPLTSTPNFVVEVIKNDDGKKALVLDCHYPEDEAESDIFSIREVSFQSTGESEWKDTNYTLNTDSLDWALYDHLMDFLADRGVDNTFADELVELSTALEHQEYITFLEDLKSFVKSQ

General information: TITO was launched using: RESULT: Template: 1P32.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 450 6715 14.92 44.47 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 14.92 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_1P32.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P32-query.scw PDB file : Tito_Scwrl_1P32.pdb: