Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSIVAIKGFNDVLPTQTAAWRRLEQHLASLMDAYGYQQIRLPIVEQTGLFKRAIGDATDIVEKEMYTFFDKGNPPESLTLRPEGTAGCVRALVEHNLLRGATPRVWYMGPMFRYEKPQKGRYRQFHQFGVETFGVATPDIDAELIMLTARLWKRMGVDHMVQLELNTLGETDERTEYRNALVAFLNEHKDALDEDSQRRLTTNPLRILDSKIESTQKILENAPKLHDFLKEDSLSHFQQLQDYLTAAGIKFVINQKLVRGLDYYNKTVFEWTTTALGSQGTVCAGGRYDGLVGQLKGKADQSVPAVGFAMGMERLLLLLEQVE---QAEIVRHCEAFLVAEPA-YQSKALVLAEQLRDQLEAANSNIRIKTG-SQSSMKSQMKKADQAGAVYAIILGEREWEAQQLAVKELATAEQSQVALAELVPFLIEKFTK 1HTT Chain:C ((3-410)) --NIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFEDRNG--DSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEHVTLELNSIGSLEARAN---------------------------------------------------YLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTN----QGTVCAGGRYDGLVEQLGGRA---TPAVGFAMGLERLVLLVQAVNPEFKADPV--VDIYLVASGADTQSAAMALAERLRDEL----PGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEVANGTAVVKDLRSGEQTAVA--------------

General information: TITO was launched using: RESULT: Template: 1HTT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1641 -124056 -75.60 -356.48 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : -75.60 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_1HTT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HTT-query.scw PDB file : Tito_Scwrl_1HTT.pdb: