Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIENPIKRRPTRKIRVGSVYVGGDAPISVQSMTNTETCDVDATVAQIERCVDAGADIMRVSVPSMEAAEAFGAIRKRV-----SVPLVADIHFDHRIALA---VADYGADCLRINPGNIGS----DQKVREVVAAARHHGISMRIGVNAGSLEKDLQKKYG-------------EPTGQALLESALRHIDILDRLDFH--EFKVSVKASNVFLTMDAYRLLSQQIDNPLHLGVTEAGIYRTGTVKSAIALGGLLMEGIGDTMRISLAAEP----EDEIKIGFDILKSLGLRSNGINFIACPSCSRQEFN-VIQVMQALEERLED----------IRTPMDVSVIGCKVNGPGEAKEADIGVVGA-APR---SLVYRNGEKSHLIDTNQLVDEIETMVRQRVQELEEAKSKEIIRSSS 4S23 Chain:B ((2-405)) ----EGMRRPTPTVYVGRVPIGGAHPIAVQSMTNTPTRDVEATTAQVLELHRAGSEIVRLTVNDEEAAKAVPEIKRRLLAEGVEVPLVGDFHFNGHLLLRKYPKMAEALDKFRINPGTLGRGRHKDEHFAEMIRIAMDLGKPVRIGANWGSLDPALLTELMDRNASRPEPKSAHEVVLEALVESAVRAYEAALEMGLGEDKLVLSAKVSKARDLVWVYRELARRTQAPLHLGLTEAGMGVKGIVASAAALAPLLLEGIGDTIRVSLTPSPKEPRTKEVEVAQEILQALGLRAFAPEVTSCPGCGRTTSTFFQELAEEVSRRLKERLPEWRARYPGVEELKVAVMGCVVNGPGESKHAHIGISLPGAGEEPKAPVYADGKLLTILKGEGIAEEFLRLVEDYVKTRFAPK---------

General information: TITO was launched using: RESULT: Template: 4S23.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1883 1876 1.00 5.24 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 1.00 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_4S23.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4S23-query.scw PDB file : Tito_Scwrl_4S23.pdb: