Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MLLVY-TLIAIVALIVMIAKFRIYPFLVLIIVSLGLALAVGMPMDGIVKSYEAGTGKTLGHLAIVIALGSMLGKMMAESG-GAERIAIALIKWFGEKHIHWAMMFIALIV-GLPVFFEVGFVLLIPIA-FNIAKRTGKSLLIVGLPMV-AGLSVVHGLIPPHPAALLAVQAYHADIGKTIMYSLLVGVPTAVVAGPLYALWINKYVKLPENNPL-AKQFVE---ADANNTRELPSFGIT-LFTIMLPVALMLVGSWADVFFAPKTFPNELLRFIGTSDIALLIAVLV-SFITFGTMQGFNREQIEKFCGGCLASIAGILLIVGAGGGFGGILRDSGISNEIVSTALKANLSPLLLGWLVAAIIRLATGSATVAMATACSIVAPIAAAAGVVVRPELLVLATGSGSLIFSHVNDAGFWLIKEYFGMTVGQTLKTWSVLETIISVLGLSFTLLLSAVL 4RUW Chain:A ((53-438)) RRDLRVMTWNIHTAVAPDAPGVVDEPRIAAVIRAEAPDVVV---LNE-VHRDAPGPG-SHGD------QPARLAELLAADGYVHTWFGLTEVDLPHEGAV-LPGSSNGNVVMSRHPFVGGGVVVPLPNENYEPGGKLRRSLLTVTVDVPGLGDVVVHATHLSTPGSAVLVEDQKEQL-RIVLDHVDARVP-SVLAGDLNIWTTDVPTQPYSQNNLMQSWIAEDHLADTWRQVNDPGAGPTMTASYGRPESPHPDRR-IDYVFATPAFDVVAGHVSLVDRFASDHLGVVMDLRLGG------------------APVAARTVLAGEDGLDGWAQLTASRPGRLRLSVCKNRGQADDDGTAVRAVLRNRAG-------------------------------------VALRTVTDSGTS-RDRCTVETWRGALPPGARLEACLVGADGTILASRTETL

General information: TITO was launched using: RESULT: Template: 4RUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2325 -129572 -55.73 -346.45 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -55.73 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.089

(partial model without unconserved sides chains): PDB file : Tito_4RUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RUW-query.scw PDB file : Tito_Scwrl_4RUW.pdb: