Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSFMQQSLKMKKILMMSMCGALLLTGCNKTSQQDQTVSKEQKSTAQKTTEQRDSSCLQIMVAMHT----INQNSKIEDLNQINEKLKTCVPSLKSEEQLKLIEASTAMYQRFLKQDYTDKTARAFEAFGYAVLEQKQDLKKVIQSQKK----LFAQLSPRDQYLLQHEGQAYIELLYQGEGMFTYRRQPNYLIDVFSKALPADQKEFLSRMAKDNQDIFYNDGALAVSWKELTERALFWEKFIQKYPKSYFINDAKLLFNEYRYFIFFGLDNTPVSNEYAPNTWFDPDALQQIRFLSTQSQSSLAKPAQKFLKFIATPVDER-NKQFKIDLTDENGQKKSTYQIVHEQLEQLLKFDSPWNTEIYRDCHIDAVCIDTN- 5JK0 Chain:A ((2-363)) -MKHPLEELKDPTENLLLWIGRFLRYKCTSLSNSQVKDQNKVFECLNELNQACSSSQLEKVCKKARNAGLLGINTYALPLLKFHEYFSK--ARLIAFNSLKNI--DEVMLAEFLSVYTGGLSLATKKNYRIALLGLFSYIDKQNQDENEKSYIYNITLKKLPTHLNNEELEKFLESIDKIEMSAKVRARNRLLIKIIVFTG-MRSNEALQLKIKD---FTLENGCYTILIKGKGDK--YRAVMLKAFHIESLLKEWLIERELY-----------PVKN----------DLLFCNQKGSALTQAYLYKQVERIINFAGLRREKNGAHMLRHSFATLLYQKRHDLILVQEALGHASLNTSRIYTHFDKQRLEEAASIWEEN

General information: TITO was launched using: RESULT: Template: 5JK0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1295 30438 23.50 90.32 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 23.50 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_5JK0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JK0-query.scw PDB file : Tito_Scwrl_5JK0.pdb: