Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKITTAIVLSMSGLIATTAMAAPHDHYHHQGYETKTVKVIHKDDHANIWRAGQVVPHQYQHPRYVI-DYRSHQKLTKPGRYQKWYKVNGNYVLVNEREFGLSRI 4EW5 Chain:A ((39-94)) ---------------------------------------------IAKNLARGKPLPPGIAK--KTVPASMLG-QLPYYP-GYEWKIVGDNLVLIALSTAVVTAI

General information: TITO was launched using: RESULT: Template: 4EW5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 168 -11085 -65.98 -201.54 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -65.98 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_4EW5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EW5-query.scw PDB file : Tito_Scwrl_4EW5.pdb: