Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATP------------FWYNTALHLLKPFYRWRIKRRAESLELYQQECLERFGPFEAP-KNVKAIWFHAVSVGETNAAQPLIEYYLKL-GQPVLVTNTTKTGQARAKSLFLKEPYLDLFQAVYLPVDQKPLLKKFFELY-QPKLLALVETELWPNLI---DQAKLQHVPCLLLNARLSEKSAKGYGKVSGLTAGMLKQLDWVLAQDSATRQRYVELGLDEHKSQVVGNIKFDIHAPEAFIKQAAQLRQQ-WYLENRQVVTIASTHAPEEQQ-ILEAFAPYLNSDRELVCIVVPRHPERFDEVFEICQNLNLITHRRSMGQSIHASTQVYLADSMGELWLWYALS-QVCFVGGSLNEPGGGHNILEPMVLNVPTVVGPRYFNFQTIVDEFIDENAVLIAQDAQQVVDIWLACLAEPEATEQLVAQAHKVLQRNQGSLQKHIGVINRYLAEKS 4RAP Chain:A ((10-391)) ITPPDTPTQAGPENIFYDFNDGARVLLPEGKWHVRLLDADSENILFCCDVDKGWVTSSKKYFVRFRIQVFRQGAT----PLLDETLKLKDRPVLISFPTGT----LGDLLGWFPYAERFQSLH---------KCRLECTMSQDIIDLLAPQ-YPQIQFSTPDKPRTVAPYATYRVGLYFGGDTNNQPVDFRKVGFHRSAGYILGVDP--REAPVRLDLSAPRV---------IAAPYVCIATQSTCQAKYWNNGTGWSEVIAHLKSLGYRVMCIDRDAHY---GQGFVWNHIPWGAEDFTGKLPLQERVNLLRH-----------ASFFIGLPSGLSWLAWATRIPVVLISGF---------SLPNSEFYTP----WRVFNSHGCYGCWDDTSLNFDHHDF-----LWCPRHKNTDRQFECTRLI---------TGAQVNGVINKLHRSLT

General information: TITO was launched using: RESULT: Template: 4RAP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1945 -8392 -4.31 -23.31 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -4.31 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_4RAP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RAP-query.scw PDB file : Tito_Scwrl_4RAP.pdb: