Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIEIRTDKNIHNSERLITYVRAELTQEFQRHSERITHFSVHFSDENGDKGGDKDIHCMIEARPSGLKPVAVHHKAGNIDASIHGAIEKLKRSLEHTFEKKEHPRGGQ-------------PEFIDDEV 1L4S Chain:A ((1-112)) -TMNITS-KQMEITPAIRQHVADRLAK-LEKWQTHLINPHIILSKEPQG----FVADATINTPN---GVLVASGKHEDMYTAINELINKLERQLNKLQHKGE------ARRAATSVKDANFVEEVEEE-

General information: TITO was launched using: RESULT: Template: 1L4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 345 -52141 -151.13 -526.68 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -151.13 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_1L4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L4S-query.scw PDB file : Tito_Scwrl_1L4S.pdb: