Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRIARIAVATYMGLAVNMGLAVNAVQAATTFLNVSYDPT----REFYQEYNQAFGKFWKQRTGQDVDFKQSHGGSGKQARAVATGLEADVVTLALANDIDEIVKAGFIQPNWQKEFP-NNSAPYTSTVVFLVRKGNPKNIRDWNDLTKPGVEIITPNPKTGGAPRWIYLSAWGYALKQPGGNDAKAKELVKKLYHNVNVLDLGA-RGSLTTFAERGIGDVLLSWENEALLATKGLDKDKYEIVYPSIS-IL--AEPSV-AIVDKTVDKDGNRTLAKGYLNFLYSPLGQELAAKHYFRPRNPQVAAKYAAQFPKIKLFTINDVFGGWAKAQKTHFANGAIFDQIYDGKQ 1SBP Chain:A ((2-308)) ----------------------------DIQLLNVSYDPTRELYEQYNKAFSAHWKQE----TGDNVVIDQSHGGSGKQATSVINGIEADTVTLALAYDVNAIAERGRIDKNWIKRLPDDSAP-YTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAWGYALHHNNNDQAKAEDFVKALFKNVEVLDSGARG-STNTFVERGIGDVLIAWENEALLATNELGKDKFEIVTPSESILAEPTVSVVDKVVEKK----DTKAVAEAYLKYLYSPEGQEIAAKNFYRPRDADVAKKYDDAFPKLKLFTIDEVFGGWAKAQKDHFADGGTFDQIS----

General information: TITO was launched using: RESULT: Template: 1SBP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1764 79714 45.19 268.40 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 45.19 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_1SBP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SBP-query.scw PDB file : Tito_Scwrl_1SBP.pdb: