Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVLRVLVTT------TALLAAGAAMAD-----EAVVHDSYAFDKNQLIP------VGARAEVGTTGYGG------ALLWQANPYVGL------------------ALGYNGGDISWTDDVSVNG----------TKYDLDMDNN---------NVYLNAEIRPWG---------ASTNPWAQGLYIAAG--AAYLDNDYDLA-KRIG-NGDTLSIDGKNYQQAVPGQEGGVRG---KMSYKNDIAPYL-----------GFGFAPKISKNWGVFGEVGAYYTGNPKVELTQYNLASVTGNPTSA----QDAVDKEANEIRNDNKYEWMPV-GKVGVNF-----YW---- 5TZJ Chain:A ((1-349)) MKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWINALGDFIQAVP-ERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGID

General information: TITO was launched using: RESULT: Template: 5TZJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1366 20895 15.30 84.25 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 15.30 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.100

(partial model without unconserved sides chains): PDB file : Tito_5TZJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TZJ-query.scw PDB file : Tito_Scwrl_5TZJ.pdb: